by Zongwen Li, Chunliu Li, Lianlian Du, Yan Zhou, Wei Wu
We previously demonstrated that human chorionic gonadotropin ? (hCG?) induced migration and invasion in human prostate cancer cells. However, the involved molecular mechanisms are unclear. Here, we established a stable prostate cancer cell line overexpressing hCG? and tested hCG?-triggered signaling pathways causing cell migration and invasion. ELISA showed that the hCG? amount secreted into medium increased with culture time after the hCG?-transfected cells were incubated for 3, 6, 9, 12 and 24 h. More, hCG? standards promoted MAPK (ERK1/2) phosphorylation and increased MMP-2 expression and activity in both dose- and time-dependent manners in hCG? non-transfected cells. In addition, hCG? promoted ERK1/2 phosphorylation and increased MMP-2 expression and activity significantly in hCG? transfected DU145 cells. Whereas ERK1/2 blocker PD98059 (25 ?M) significantly downregulated phosphorylated ERK1/2 and MMP-2. Particularly, hCG? promoted cell migration and invasion, yet the PD98059 diminished the hCG?-induced cell motility under those conditions. These results indicated that hCG? induced cell motility via promoting ERK1/2 phosphorylation and MMP-2 upregulation in human prostate cancer DU145 cells.
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Human Chorionic Gonadotropin ? Induces Migration and Invasion via Activating ERK1/2 and MMP-2 in Human Prostate Cancer DU145 Cells
Syndicated from:PLoS ONE
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